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High performance packed chromatography columns
Instrument Description A packed column is a chromatography column packed with a stationary phase and is a key element in both gas and liquid chromatography. The separation of the mixture is achieved through the interaction between the stationary and mobile phases. Con...
Description
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Description of the device
A packed column is a chromatography column packed with a stationary phase and is a key element in both gas and liquid chromatography. The separation of the mixture is achieved through the interaction between the stationary and mobile phases.
Design features and operating principle
Basic design: The column is a tube, usually made of stainless steel, filled with a granular stationary phase (for example, an adsorbent or an inert carrier coated with a liquid phase). The ends of the tube are covered with glass wool.
Typically the internal diameter is 2–5 millimeters, and the length is from 0.5 to 10 meters.
The stationary phase may be a solid adsorbent (gas-solid-phase chromatography) or a liquid stationary phase supported on an inert carrier (gas-liquid chromatography).
Separation principle: the sample is carried by a flow of carrier gas or mobile phase through a filler layer. Different components of a mixture are retained in the column for different times due to differences in adsorption capacity or partition coefficients, resulting in their sequential exit from the column. The stronger the interaction of a component with the stationary phase, the longer the retention time and the later it elutes.
Typical Applications
Analysis of permanent gases, lower hydrocarbons, moisture content and other routine samples.
Separation and analysis of nitrobenzenes, benzenes and their derivatives in industrial wastewater. Preparative chromatography for the isolation of pure substances.
They have proven themselves in the analysis of samples with high load and complex matrix, where large column capacity is required.
Key differences from capillary columns
Comparison of characteristics:
Column efficiency: The separation efficiency of the packing column is lower, it is suitable for simple mixtures;
Capacity: The packing column has a large capacity and can handle relatively “dirty” samples.
Operating costs:
Filling columns are easy to make, you can fill them yourself, they are inexpensive, and the filler can be replaced if dirty.
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